DoGENBio efficacy kits — Efficacy data for a new ingredient, from one kit set

Kits by study aim

This table shows what each kit measures, in which cells, and what the result does not prove on its own. You choose the measurement that fits your study aim. The kit gives you the number.

Study aim	What the kit measures	DoGENBio kit	Typical cells or sample	Note
Working concentration range	Cell viability (dehydrogenase activity) and membrane damage (LDH release) at each concentration, under your assay conditions	EZ-CytoxEZ-LDH	HaCaT keratinocytes, human dermal fibroblasts, B16F10, RAW 264.7	Cell test only. It does not prove skin irritation or a safe concentration for people. Those need OECD test guidelines (for example TG 439 on reconstructed epidermis) and the market's safety assessment.
Antioxidant activity	Radical scavenging and total antioxidant capacity in a cell-free test. Enzyme activity, glutathione redox ratio and lipid peroxidation in cell or tissue lysates.	DPPHABTSTAC (Trolox)SODCatalaseGPxGSH/GSSGTBARS	Extracts and fractions; HDF or HaCaT lysates after H₂O₂ or UV treatment	Cell-free test. It does not prove an antioxidant benefit on skin. A cell-free result does not predict the effect in a formulation or in a living body.
Collagen-related	Total collagen, measured as hydroxyproline, in cells, tissue or medium	EZ-Total CollagenEZ-Cytox	Human dermal fibroblasts, with or without TNF-α or UV treatment	Does not show new collagen synthesis. To separate synthesis from breakdown you need a labelled or gene-expression method.
Inflammatory response	Nitrite (Griess) in the medium of stimulated macrophages. Cell viability at the same concentrations, to make sure the drop in nitrite is not caused by cell death.	EZ-Nitric OxideEZ-Cytox	LPS-stimulated RAW 264.7	Does not prove an anti-inflammatory effect or mechanism. Nitrite is one marker only. It is usually reported together with cytokine data.
Pigmentation studies	Cell viability at each concentration, so your melanin and tyrosinase tests run at non-toxic concentrations	EZ-Cytox plus your own melanin or tyrosinase method	B16F10 melanoma cells, α-MSH stimulated	Does not prove a brightening effect. The pigment result comes from your own assay. Effects on skin colour need clinical testing.
Hair-research cell models	Cell viability and ATP level of dermal papilla cells	EZ-CytoxEZ-ATP	Human hair follicle dermal papilla cells	Does not prove hair growth or an anti-hair-loss effect. Those need hair follicle organ culture or clinical studies.
UV exposure models	Viability of keratinocytes or fibroblasts, and lipid peroxidation, after UVA or UVB exposure	EZ-CytoxTBARSCatalase	HaCaT, HDF	Does not prove photo-protection or SPF. Those have their own standard test methods.
Lipid and glucose (nutraceutical)	Triglyceride, free glycerol, free fatty acid, cholesterol and HDL/LDL levels in lysates or serum. Glucose level in medium (glucose used by the cells) or in lysate.	TriglycerideFree GlycerolFree Fatty AcidGlucoseTotal CholesterolHDL / LDL-VLDL	3T3-L1 adipocytes, HepG2, C2C12; serum in animal studies	Does not measure glucose uptake into cells (that needs a tracer method). Does not prove weight or metabolic health effects.
Enzyme and cofactor markers	Acetylcholinesterase activity; NAD⁺/NADH concentration and ratio	EZ-AChEEZ-NAD/NADH	Extracts; SH-SY5Y or tissue lysates	Does not prove a benefit for cognition or ageing.
Fermentation and probiotics	Ethanol and lactate produced, glucose left, and SOD activity in broth or fermentate	EZ-EthanolEZ-LactateGlucoseSOD	Culture broth, fermentate	Does not prove probiotic health effects.
Vitamin C content	Ascorbic acid level in a raw material or product	EZ-Ascorbic Acid	Fruit extracts, formulations	Not enough for a label claim. That needs a validated analytical method (for example HPLC) in the target market.

Cell viability kitsCell-free antioxidant kitsNitrite kitEnzyme, metabolite and content kits

All kits are read by colour on a standard 96-well plate reader. The ATP, catalase, H₂O₂/peroxidase, free fatty acid, glycerol, glucose, cholesterol and triglyceride kits can also be read by fluorescence. Kits are for research and product development. They do not replace regulatory safety testing or the tests needed to support a claim.

Four kit panels, one for each study type

A panel is a set of kits shipped together with one protocol sheet. You run it in your lab and get one comparable data set. Start with the panel that matches your study aim. Add single kits later if you need them.

Skin efficacy panel

Serums, creams, masks, active ingredients for skin

First find the working range on keratinocytes and fibroblasts. Then measure antioxidant capacity, total collagen and nitrite in that range. These are the cell-test results usually put in the ingredient file for antioxidant, firming and soothing products.

EZ-Cytox, DPPH, ABTS, TAC, EZ-Total Collagen, EZ-Nitric Oxide

Antioxidant defence panel

Botanical extracts, marine and fermented active ingredients, anti-pollution claims

Measures what the ingredient does inside cells under stress: enzyme activity, glutathione redox state and lipid peroxidation. Total antioxidant capacity is the main number.

SOD, Catalase, GPx, GSH/GSSG, TBARS, TAC, H₂O₂/Peroxidase

Hair and scalp panel

Anti-hair-loss and scalp-care active ingredients

Measures viability and ATP level of dermal papilla cells, plus nitrite for scalp inflammation models. The 2026 Theranostics alopecia study used EZ-Cytox to measure viability of dermal papilla cells.

EZ-Cytox, EZ-ATP, EZ-Nitric Oxide, EZ-LDH

Metabolic and nutraceutical panel

Weight, lipid, glucose, fermented and probiotic products

Measures lipid build-up and breakdown (lipolysis) in adipocytes, glucose use by liver and muscle cells, and fermentation products in broth. EZ-Cytox sets the working range.

Triglyceride, Free Glycerol, Free Fatty Acid, Glucose, Lactate, Ethanol, Cholesterol, EZ-Cytox

How each kit works, its standard curve and its pack sizes are in the assay kit brochure (PDF). Panel price is quoted per project. Storage differs by kit: EZ-Cytox, collagen and nitric-oxide kits at 0–4 °C; several enzyme and metabolite kits at −20 °C. Each panel ships with a storage card for every SKU.

How EZ-Cytox compares to CCK-8

Most efficacy labs find the working range with a cell viability / cytotoxicity assay kit. EZ-Cytox is a WST-based reagent. It is used the same way as the kit you use now. Add 10 µL per 100 µL well. Incubate 30 minutes to 4 hours at 37 °C. Read at 450 nm. No washing. You can still use the cells in the same well for LDH or Western blot. To check that it gives the same result on your cells and extracts, run one plate: half with your current kit, half with EZ-Cytox.

Plant extracts and coloured or reducing ingredients can disturb any colour-based assay, including WST. Run a reagent blank without cells for each extract concentration. For an important result, confirm it with a second test such as EZ-LDH.

Every panel above uses EZ-Cytox for the working range. So the working-range data in all your files (antioxidant, collagen, hair) come from the same lot-tested product and protocol.

The step-by-step protocol comparison, the switching FAQ and the one-plate test are on the research and distributor page. Prices are quoted by country, pack size and volume, with the panel or alone. Compare the same pack size and the total delivered cost, including freight, duties and taxes.

Tests per pack	Dojindo CCK-8	DoGENBio EZ-Cytox
500	CK04-05, 5 mL	EZ-500, 5 mL × 1
1,000	CK04-11, 5 mL × 2	EZ-1000, 5 mL × 2
3,000	CK04-13, 5 mL × 6	EZ-3000, 5 mL × 6
10,000	CK04-20, 100 mL	EZ-BULK150, 25 mL × 4

Matching pack sizes. Prices are not printed here. The same kit sells at a different price in each country. So we quote EZ-Cytox and the panel by country, against the price you pay today. Tell us your current price per pack in the request form. The quote comes with the evaluation panel.

Standard curves compared with competitor kits

These numbers are from the DoGENBio catalogue. Each kit was run next to a widely used competitor kit under the same conditions. The full curves, conditions and raw data ship with the panel.

1.9×DPPH slope

Absorbance change per µg/mL Trolox at 517 nm: −0.0041 (EZ-DPPH) vs −0.0022 (competitor B). R² 0.997 vs 0.946.

+10%Glutathione slope

Absorbance per µM GSSG at 412 nm: 0.874 (EZ-Glutathione) vs 0.796 (competitor O). R² 0.995 vs 0.994.

1.5×Ascorbic acid slope

Absorbance change per nmol/well: 0.119 (EZ-Ascorbic Acid) vs 0.081 (competitor B). R² 0.999 vs 0.999.

The printed catalogue does not name the competitors. The technical data sheet names them, on request. A steeper standard curve means more signal per unit of analyte on your reader. This is only one part of sensitivity. That is why we offer the panel for you to test on your own samples.

Published studies that used DoGENBio kits

These published studies used a DoGENBio kit for one of their measurements. Each card names the kit and the measurement, and links to the paper.

Hair

Theranostics 2026

DKK3–CKAP4 signaling drives fibroimmune remodeling and hair follicle miniaturization in androgenetic alopecia

EZ-Cytox: viability of human dermal papilla cells

Anti-aging, Thai botanical

Antioxidants 2021

Protective effect of polymethoxyflavones isolated from Kaempferia parviflora against TNF-α-induced human dermal fibroblast damage

EZ-Cytox: viability of HDF. Black ginger, a Thai ingredient

Brightening

Journal of Extracellular Vesicles 2020

Anti-melanogenic effects of extracellular vesicles derived from plant leaves and stems in mouse melanoma cells

EZ-Cytox: viability of B16F10. Melanin measured by the authors' own method.

Brightening

Journal of Dermatological Science 2021

A natural compound harmine decreases melanin synthesis through regulation of the DYRK1A/NFATC3 pathway

EZ-Cytox: cell viability. Melanin measured by the authors' own method.

Collagen

Scientific Reports 2020

Near-infrared-emitting nanoparticles activate collagen synthesis via TGFβ signaling

EZ-Cytox: viability of HDF and HaCaT. Collagen measured by the authors' own method.

Elasticity

Journal of Ginseng Research 2020

Effects of red ginseng on the elastic properties of human skin

EZ-Cytox: cell viability in the cell-culture part of the study

Sun care

Nutrients 2020

Effect of konjac glucomannan on the reconstitution of the dermal environment against UVB-induced damage

EZ-Cytox: cell viability after UVB exposure

Soothing

Phytomedicine 2021

Sophoricoside from Styphnolobium japonicum improves experimental atopic dermatitis in mice

EZ-Cytox: viability in the cell experiments

Wound, botanical

Journal of Ethnopharmacology 2017

Wound healing effects of deoxyshikonin isolated from Jawoongo: in vitro and in vivo studies

EZ-Cytox: viability of HaCaT and HUVEC

Cosmeceutical

Antioxidants 2020

Microbial production of retinyl palmitate and its application as a cosmeceutical

EZ-Cytox: viability

Antioxidant enzymes

Antioxidants 2019

Anti-oxidant activity of gallotannin-enriched extract of Galla Rhois can associate with the protection of the intestinal barrier

EZ-Catalase and EZ-SOD kits: enzyme activities

Lipolysis

Frontiers in Pharmacology 2019

Ginsenoside Rb1 induces β3-adrenergic receptor-dependent lipolysis and thermogenesis in 3T3-L1 adipocytes

EZ-Free Fatty Acid kit: fatty acids released by 3T3-L1 cells

See the full list at dogenbio.com › Reference Library. Or ask us for the list sorted by study area and cell type.

The evaluation panel

Tell us your ingredient and your study aim. You run the panel in your own lab. DoGENBio supplies the kits and a protocol, so you get one comparable data set. Evaluation panels are available within limits that we confirm by email. We do not offer a testing service.

Describe the study

Tell us the ingredient and the study aim (antioxidant, collagen, inflammation model, pigmentation, hair, metabolic, fermentation). Also tell us the cells or samples your lab already uses.

We choose the kits

We choose the kits for your study: one ingredient, six concentrations, three repeats, with blanks, controls and standard curves. You get a written protocol and plate maps.

You run it

First find the working range. Then measure the effect and the marker in that range. All tests are read by colour on your plate reader. After cell culture and treatment, each measurement takes 30 minutes to 2 hours.

We help with the analysis, if you ask

Send us your result files. We send back curves, IC50 values and a one-page summary for your ingredient file. We also send a methods text for your report. Your raw data stays yours. We do not share it.

Request the panel or a quote

What do you need

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Cytotoxicity kit you use now

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About your ingredient and timeline

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Who this page is for

ODM and OEM efficacy labs

Manufacturers that run cell tests for hundreds of brand customers. They test many ingredients every month.

Ingredient suppliers

Suppliers of botanical, marine and fermented ingredients. They need a data file to support claims before a brand will use the ingredient.

Brand R&D

In-house teams that find the working range and screen ingredients before sending the final product to a clinical CRO.

Nutraceutical and food R&D

Lipid, glucose, fermentation and antioxidant measurements for functional foods and cosmetic products.

CROs and testing laboratories

Labs that offer routine cytotoxicity and antioxidant tests. Reagent cost per sample matters in every quote.

University cosmetic-science groups

Labs that publish. These kits are already accepted in the journals listed above.

Efficacy data for a new ingredient,
from one kit set.

Three measurements for one ingredient