EZ-Cytox by DoGENBio — Same protocol as CCK-8. Test it on one plate.

Three reasons to test EZ-Cytox, even if CCK-8 works well for you

We do not ask you to trust a claim. We ask you to read one plate.

A price made for grant-funded labs

We make EZ-Cytox in our own facility. So we can price it for labs that pay for reagents from grants and teaching budgets. Reagent prices differ a lot by country and reseller. So we do not print one price for every market. We quote by country, pack size, volume and terms. You can ask for a quote with the test kit or on its own. Telling us what you pay today is optional.

Same steps as CCK-8: add, incubate, read

EZ-Cytox is a water-soluble tetrazolium (WST) reagent. It comes as a sterile, ready-to-use premix. Add 1/10 volume, incubate, read at 450 nm. No solubilisation step. No wash. Works with suspension or adherent cells, in 6- to 384-well plates. The handling is the same as CCK-8. How the signal behaves on your cell lines, densities and compounds is something you check on the bench. A datasheet cannot tell you that. That is the point of the split plate.

You talk directly to the maker

DoGENBio develops, makes and fills EZ-Cytox in Seoul. Each lot is tested on live cells and released with a certificate of analysis. Technical questions go to the people who make the reagent. We commit to reply within 48 hours on business days. Your question does not pass through three layers of distributors.

Step-by-step comparison: CCK-8 vs EZ-Cytox

This table shows which steps are the same when you run a CCK-8 protocol with EZ-Cytox. It also shows what you should check on your own cells first.

Step	CCK-8 (Dojindo CK04)	EZ-Cytox (DoGENBio)	Difference
Chemistry	Water-soluble tetrazolium (WST-8). Cell dehydrogenases turn it into an orange formazan that dissolves in the medium	Water-soluble tetrazolium (WST). Cell dehydrogenases turn it into an orange formazan that dissolves in the medium	None (same principle)
Reagent volume	10 µL per 100 µL well (1/10 volume)	10 µL per 100 µL well (1/10 volume)	None
Incubation	1–4 h at 37 °C, depends on cell type and number	30 min – 4 h at 37 °C, depends on cell type and number	Same range; set the time per cell line
Measurement	Absorbance at 450 nm, reference 600 nm or above	Absorbance at 450 nm, reference 600 nm or above	None
Plate formats	6- to 384-well, adherent or suspension, no wash	6- to 384-well, adherent or suspension, no wash	None
Cells afterwards	Low toxicity; cells can be used for more assays	Non-toxic premix; the same cells can be used for DNA/RNA extraction, Western blot or an LDH assay	Check for your next assay
Storage	0–5 °C, protected from light, 1 year	0–4 °C, protected from light; expiry date printed on each lot	Similar
Pack sizes	100, 500, 1,000, 3,000, 10,000 tests	500, 1,000, 3,000, 5,000, 10,000 tests (based on a 96-well plate). EZ-Cytox Plus for low cell numbers or slow-metabolising cell lines	More sizes
Catalogue number	CK04-xx	EZ-500 / EZ-1000 / EZ-3000 / EZ-5000 / EZ-BULK150 / EZ-3000P	Changes

CCK-8 values are from the Dojindo CK04 technical manual. EZ-Cytox values are from the DoGENBio product manual and catalogue. Test counts for both assume 10 µL per well. Blanks, controls and repeats also use reagent. Both reagents are for research use only.

Same handling does not mean same numbers. Any two WST reagents can differ in reaction speed, dynamic range and interference from coloured or redox-active compounds. Normalising to an untreated control makes the data the same in format, not always in value. Run a bridging plate for each cell line and each type of treatment. Keep it in the study file. Do not switch reagents in the middle of a study without recording the bridge.

Which pack replaces which, and how we quote

This table maps Dojindo catalogue numbers to DoGENBio catalogue numbers. It is for lab managers and distributors. We do not print prices here. The same kit sells at very different prices in Kumamoto, Maryland and Bangkok. So we quote EZ-Cytox by country, against what you pay today. The quote comes with the test kit.

Tests per pack	Dojindo CCK-8 (CK04)	DoGENBio EZ-Cytox	Note
100	CK04-01, 1 mL	Test kit only	Sample size only; not a stock item
500	CK04-05, 5 mL	EZ-500, 5 mL × 1	Same volume
1,000	CK04-11, 5 mL × 2	EZ-1000, 5 mL × 2	Same volume
3,000	CK04-13, 5 mL × 6	EZ-3000, 5 mL × 6	Same volume. EZ-3000P is the more sensitive Plus version
5,000	CK04-15, 5 mL × 10	EZ-5000, 25 mL × 2	Fewer vials to open
10,000	CK04-20, 100 mL × 1	EZ-BULK150, 25 mL × 4	Bulk pack in four vials, so you open each bottle less often
LDH release	Cytotoxicity LDH Assay Kit-WST (CK12), read at 490 nm	EZ-LDH, DG-LDH500 / DG-LDH1000, read at 450 nm	Runs on the same plate as EZ-Cytox. One filter for both readings

Dojindo catalogue numbers and volumes are from dojindo.com (September 2026). DoGENBio volumes are from the EZ-Cytox catalogue. Other kits map the same way: SOD Assay Kit-WST to EZ-SOD, GSSG/GSH Quantification Kit (G257) to EZ-Glutathione, Glucose Assay Kit-WST (G264) to EZ-Glucose, Lactate Assay Kit-WST (L256) to EZ-Lactate, NAD/NADH Assay Kit-WST (N509) to EZ-NAD/NADH, Griess reagent kit to EZ-Nitric Oxide. Dojindo has no equivalent for the EZ-Western ECL substrates, the collagen kit or the total antioxidant capacity kit.

How we quote: by country, pack size, yearly volume and terms. Prices are ex-works Seoul, with freight and duties shown separately. Once a distributor is appointed, quotes come through the distributor. You can ask for a quote with the test kit or without a sample. Telling us what you pay today is optional. It helps us reply in your local terms. Compare like with like: the same pack size, and the total delivered cost including freight, duties and taxes.

Publications that used EZ-Cytox

EZ-Cytox has appeared in the Methods sections of papers since 2010. DoGENBio's public reference library lists 764 entries. That is 634 different papers, after removing papers listed under more than one product. Each card below links to the paper.

Signal Transduction and Targeted Therapy 2026

Personalized PK–PD guided therapy via an iPSC-derived multi-organoid platform in NF1-mutant breast cancer

EZ-Cytox (EZ-1000), viability of intestine, liver and kidney organoid models 2025 JIF 81.2

Nature Communications 2019

Ciclopirox inhibits Hepatitis B virus secretion by blocking capsid assembly

EZ-Cytox, cytotoxicity of the compound in HepG2-derived cells. A 2021 Nature Communications paper used the EZ-ATP kit.

Biomaterials 2021

Systemic delivery of CRISPR/Cas9 to hepatic tumors for cancer treatment using altered tropism of lipid nanoparticles

EZ-Cytox, nanoparticle cytotoxicity in Huh7 cells. The library has 17 papers from Biomaterials and Biomaterials Science.

Cell Death & Disease 2021

B7-H3 regulates osteoclast differentiation via type I interferon-dependent IDO induction

EZ-Cytox, viability of osteoclast precursor cultures. 11 papers across Cell Death & Disease, Differentiation and Discovery.

Molecular Therapy 2019

CRISPR-Pass: gene rescue of nonsense mutations using adenine base editors

EZ-Cytox for viability and EZ-Western Lumi Pico for the blots, in the same study.

Theranostics 2026

DKK3–CKAP4 signaling drives fibroimmune remodeling and hair follicle miniaturization in androgenetic alopecia

EZ-Cytox, viability of human dermal papilla cells, 1 h incubation, 450 nm.

Cancer and oncology340

Skin, cosmetics, wound79

Inflammation and immunity63

Bone, cartilage, regeneration51

Metabolism, diabetes, liver50

Neuroscience48

Cardiovascular and renal34

Natural-product studies (all fields)220

Each library entry is counted under one main topic (September 2026). The natural-product count is a separate tag and overlaps with the topics above. Browse the full list at dogenbio.com › Reference Library. Or ask us for the spreadsheet sorted by field and cell line.

Common questions from CCK-8 users

Is the chemistry the same as WST-8?

Both are water-soluble tetrazolium salts. Cell dehydrogenases turn them into an orange formazan that dissolves in the medium. Both are read at 450 nm. DoGENBio labels EZ-Cytox as a WST reagent. The exact tetrazolium and formulation are DoGENBio's own. They are stated in the technical documents that come with the test kit. At the bench, what matters is that the reagent volume, incubation time, wavelength and plate handling are the same.

Can I compare my old CCK-8 data with EZ-Cytox data?

Not automatically. Percent-of-control normalisation removes the difference in absolute OD. It does not remove differences in reaction speed, dynamic range or compound interference. Treat the split plate as a first check. Then run a bridging plate for each cell line and treatment type you rely on. For a long study, the safe choice is to finish it with the reagent you started with. Change at the next study.

What about phenol red, serum and reducing agents?

The same rules as any WST assay. Phenol red is handled by blank subtraction. Reducing agents such as ascorbate or DTT can reduce tetrazolium directly. If your compounds contain them, include a reagent blank with no cells.

Does it work with suspension cells and leukocytes?

Yes. Slow-metabolising or low-density cultures need the longer end of the incubation range. EZ-Cytox Plus (EZ-3000P) is the more sensitive version for those cases.

Can I run LDH or a Western blot on the same cells afterwards?

Yes. The premix is not toxic to the cells and needs no lysis. Many labs run EZ-Cytox and EZ-LDH on the same plate. This separates growth inhibition from membrane damage. Then they lyse the cells for protein.

How long is shipping to Bangkok, Kuala Lumpur or Singapore, and how is it packed?

Stock is held in Seoul. Each product is stored and shipped at its labelled condition (refrigerated or frozen, stated per SKU). The packaging matches that condition. Express courier to Southeast Asian hubs usually takes 3–5 days. Once a distributor is appointed for a territory, stock is held locally.

Three measurements from one set of cells

A drop in viability does not tell you why. Some labs need to separate growth inhibition from cell death, or check the energy state of the cells. They plan three measurements on cells from the same seeding.

ViabilityEZ-Cytox, WST, 450 nm

Dehydrogenase activity of living cells. The first screening measurement.

Membrane damageEZ-LDH, 450 nm, same plate

LDH released from damaged cells. Separates cytotoxicity from growth inhibition. Also the standard measurement for ADCC, CDC and NK killing assays.

Energy stateEZ-ATP, colorimetric or fluorometric

Cell ATP as an extra metabolic marker. Useful when a compound affects mitochondrial function.

Basic EZ-CytoxDuo-T EZ-Cytox + EZ-LDHDuo-V EZ-Cytox + EZ-ATPTriple all three, with one combined protocol sheet

A step-by-step protocol sheet comes with the bundle. Check three things in your own system before you use the combination for decisions. First, the run order. Second, how you sample the supernatant for LDH. Third, interference from coloured or redox-active compounds. Storage: EZ-Cytox and the LDH assay buffer at 0–4 °C; LDH substrate and ATP kit components at −20 °C.

Products a distributor can stock

One shipment covers cell analysis, protein detection and 23 metabolism and oxidative-stress kits. Each product is packed at its labelled storage condition (refrigerated or frozen).

EZ-Cytox kit box with three reagent vials

EZ-Cytox and EZ-Cytox Plus

WST cell viability, proliferation and cytotoxicity. The main product on this page.

500 to 10,000 tests (based on a 96-well plate); Plus for low cell numbers · Brochure (PDF)

EZ-LDH

WST-based LDH release assay for cytotoxicity, ADCC, CDC and NK activity. One step, about one hour.

DG-LDH500 (500 assays), DG-LDH1000 (1,000 assays)

EZ-Western ECL, five sensitivity levels

Chemiluminescent substrates from routine blots to low-femtogram targets. Also stripping buffer, gel stain, BCA and Bradford. See the guide below.

100 to 500 mL per level · Brochure (PDF)

EZ-NAD/NADH assay kit box with reagent vials

23 assay kits

Oxidative stress: SOD, GSH/GSSG, catalase, H₂O₂/peroxidase, TBARS, TAC, DPPH, ABTS, GPx. Metabolism: lactate, AChE, ascorbic acid, ATP, free fatty acid, glycerol, glucose, HDL/LDL, cholesterol, triglyceride, nitric oxide, collagen, ethanol, NAD/NADH (new in 2026).

100 to 500 assays; colorimetric, many also fluorometric · Brochure (PDF)

OEM · Private label

Your brand. Our reagents.

We can supply EZ-Cytox, EZ-LDH, the Western blot ECL substrates and the 23 assay kits under your own label. They are made and tested lot by lot in the same Seoul facility, under ISO 9001, with a certificate of analysis for every lot.

Your label, your box and your catalogue numbers.
The same reagent and the same quality checks as the DoGENBio brand.
Scope, pack formats, minimum quantities and documents are agreed per project.
One partner can combine distribution of the DoGENBio brand with a private-label line.

How it starts

Choose "OEM / private-label supply" in the request form. Tell us the products, your territory and a rough yearly volume. We reply within two working days with an OEM outline.

Ask about OEM supply

Western blot ECL: which substrate for which target

Five substrates based on one chemistry. DoGENBio's catalogue compares each one with the SuperSignal product a lab would otherwise buy. Same lysate, same antibody, exposure series side by side.

Level	Use it for	Compared with	Catalogue no. and volumes
EZ-Western	Abundant target and plenty of antibody. The low-cost blot to check if a protein is present or absent	SuperSignal West Pico	DG-W100, DG-W250, DG-W500 (100, 250, 500 mL, A+B)
EZ-Western Lumi Pico	The everyday substrate. Needs less antibody than the basic level	SuperSignal West Pico	DG-WP100, DG-WP250, DG-WP500
EZ-Western Lumi Pico Alpha	Made for imagers (LAS, ChemiDoc class). Fast signal that stays stable long enough for repeat captures	SuperSignal West Pico PLUS	DG-WPAL120, DG-WPAL250
EZ-Western Lumi La	Mid-femtogram targets. The signal lasts for hours after you add the substrate	SuperSignal West Dura	DG-WD100, DG-WD200
EZ-Western Lumi Femto	Low-femtogram targets, very little sample, or high antibody dilutions	SuperSignal West Femto	DG-WF100, DG-WF200
Supporting products	Stripping buffer DG-WSB500 (500 mL); CBB gel stain DG-GS1000 (1 L, no destain); BCA DG-BCA500; Bradford DG-BRA500; membrane tray DG-WMT8

Catalogue test conditions: HeLa lysate, Erk1/2 primary antibody at 1:1,000 to 1:5,000, anti-rabbit secondary at 1:5,000 to 1:100,000, PVDF or NC membrane, exposure series 10 s to 60 min (Lumi La up to 26 h). The comparator version and lot are stated in the catalogue. The full blot images and conditions are in the ECL brochure (PDF). Results depend on your antibody, membrane and imager. So test it the same way as the plate: run one membrane with both substrates before you switch.

The split-plate test

One plate. One afternoon. Your cells and your plate reader. You keep the data, whatever the result.

Tell us your setup

Your cell line, seeding density, the compounds or conditions you already test, your current kit and your plate reader.

We ship the kit

We send an EZ-Cytox test pack with a one-page split-plate protocol. EZ-LDH and EZ-ATP can be added on request. It ships at its labelled storage condition (refrigerated or frozen) to your lab or your distributor.

You run one plate, in your lab

Seed the whole plate the same way. Add your current reagent to columns 1–6 and EZ-Cytox to columns 7–12. Incubate both for the same time. Read at 450 nm. After your usual culture and treatment time, the measurement itself takes 30 min to 4 h.

Compare, then decide

The data is yours. If you want a second opinion, send us the reader export. We send back a short comparison (CVs, correlation, plate map). If the results do not match, tell us. That result is useful to us too.

Request a test kit or a quote, or ask about becoming a distributor

What do you need

Name

Institution or company

Work email

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Viability kit you use today

Main cell lines

What you pay per pack today (optional)

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Anything else (plate reader model, timing, volumes)

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Please do not include patient data or confidential formulation details. A general description is enough to start.

We use your details only to answer this request and, if you ask for one, to ship a test kit. We reply within two working days. No newsletter.

Request received We have sent a confirmation to your email. A person will reply within two working days. If nothing arrives, write to robin@dogenbio-global.com.

For distributors: what you get from working with the manufacturer

DoGENBio is appointing one partner per territory in Thailand, Malaysia and Singapore. Vietnam, Indonesia and the Philippines will follow. Choose "Become a distributor" in the request form. The form then shows the questions we need for a territory discussion.

Territory rights in a written agreement

Territory, yearly targets and any exclusivity are set only in a signed distribution agreement. The agreement is reviewed every year. Nothing on this page grants or promises exclusivity.

Margin for a price-led product line

Your supply price is set per territory. It depends on local market conditions, pack mix and committed volume. It leaves room for a competitive shelf price and a marketing contribution in year one. We share price lists under NDA once the territory discussion starts.

Test kits for your key accounts

We provide split-plate kits for the labs you choose. They decide on their own data, not on a sales pitch.

Reference list by field and cell line

A sorted list of the 634 published uses. Your sales team can give an oncology lab oncology papers, and a dermatology lab skin papers.

Technical support direct from the manufacturer

We reply within 48 hours on business days. This is written into the distribution agreement. Your product managers talk to the scientists who make the reagent.

Storage condition stated per product

Each lot comes with a certificate of analysis. A storage table lists the condition for each SKU (refrigerated or frozen). So nothing is shipped or stored at the wrong temperature. Shipping packaging matches each product's condition.

Fit with your current brands, discussed openly

If you do not sell a cell viability / cytotoxicity assay kit today, this line adds a new category. If you do, EZ-Cytox is aimed at price-sensitive customers: teaching labs, publicly funded groups and high-volume screening. We work out with you how it fits next to your current brand, territory by territory, before either side commits.

Three product groups in one shipment

Cell analysis kits lead. ECL substrates and the 23 assay kits ship in the same consignment, each at its labelled storage condition. They help you grow the account.

OEM and private-label supply

We can also supply EZ-Cytox, EZ-LDH, the ECL substrates and the assay kits under your own label. Scope, pack formats, minimum quantities and documents are agreed per project. See the OEM section above, or choose "OEM / private-label supply" in the request form.

Made, tested and supported in Seoul

DoGENBio started with EZ-Cytox in 2008 and was incorporated in 2014. We develop, make and fill our reagents in our own facility in Seoul. Every lot is tested on live cells before release. The company holds five patents. It is an accredited Venture, Inno-Biz and Main-Biz enterprise, with a certified corporate research institute.

ISO 9001:2015 GQ-242997ISO 14001:2015 GE-241518ISO 45001:2018 GO-240735

We supply EZ-Cytox and the assay kits to universities, research institutes and companies across Korea.

Researcher pipetting at a laboratory bench